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The 2nd reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circularly polarized luminescence. As drawn in Fig - https://www.quora.com/profile/Julie-Ann-DeSa-Lorenz. 9, the response may likewise involve electron transfer from H4B to the Fe, IIO2 intermediate
l9 As shown in Fig. 9, this might be because the H4B extreme acts as an electron sink in a later point in the response, so as to allow NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 types, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.